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Jinfeng Ni
Phone number: 0531-88363323
Fax number: 
E-mail: jinfgni@sdu.edu.cn
Title: Professor


Ph.D: 1998.04 – 2002.03, Science of Biological Resources, The United Graduate School of Agricultural Science, Gifu University, Japan.

Master: 1988.09 – 1991.06, Cell Engineering, Department of Biology, Lanzhou University, China.

Bachelor: 1984.09 – 1991.06, Zoology, Department of Biology, Lanzhou University, China.


2009.12-to present, Professor, State Key Laboratory of Microbial Technology, Shandong University

2012.09 to 2013.08, Visiting researcher in School of Chemical and Biological Engineering in Seoul National University (International Scholar Exchange Fellowship supported by Korea Foundation for Advanced Studies), Seoul, Korea.

2004.04-2009.11, Associate Professor, State Key Laboratory of Microbial Technology, Shandong University, Jinan, China.

2004.09-2006.09, JSPS researcher, National Institute of Agrobiological Science, Tsukuba, Japan, Working on directed evolation of termite cellulases.

2003.04 to 2004.03, BRAIN postdoctoral fellowship in Insect Genetics and Evolution Department, National Institute of Agrobiological Science, Tsukuba, Japan. Working on termite origin cellulases.

2002.05 to 2003.03, Research assistant in Photosynthesis Laboratory, Plant Physiology Department, National Institute of Agrobiological Science, Kannondai, Tsukuba, Japan. Involved in molecular dissection of protein interaction network in phytochrome-mediated signal-transduction.

1991.06 to 1994.09, Research assistant in Jincheng Hospital, Lanzhou, Gansu, China.


1.Termite lignocellulose-decomposing system and lignocelluolytic enzymes;

2.Molecular evolution of lignocellulolytic enzymes from termite and hyperthermostable archaea;

3.The structure and function of enzymes involving in DNA recombination and repair mechanism in hyperthermostable archaea.

Major: Bioresources, Applied Microbiology, Biochemistry and Molecular Biology Mechanism of lignocelluloses degradation in termite


1.General Program from the Natural Science Foundation of China (NSF), 2015-2018;

2.General Program from the Natural Science Foundation of China (NSF), 2013-2016;

3.A sub-project of the Major State Basic Research Development Program of China (973). 2011-2015;

4.The project from State Key Laboratory of Microbial Technology project, 2015-2016.


1.Shi X, Zhang N, Ning N, Yun C, Ni J*. (2016) Isolation and identification of xylan -degrading bacteria from fungus-growing termite hindgut. Microbiology China. 3(3): 504-9.

2.Sun X, Yang Y, Zhang N, Shen Y, Ni J*. (2015) Draft Genome Sequence of Dysgonomonas macrotermitis Strain JCM 19375T,Isolated from the Gut of a Termite. Genome Announc, 3(4), 10.1128/genomeA.00963-15.

3.Ning N, Zhang N, Wu Y, & Ni J*. (2015) Comparison of lignocellulolytic enzyme activities of the fungus-growing termite Macrotermes barneyi and the lower termite Coptotermes formosanus. Chin J Appl Environ Biol, 21(4): 678-82.

4.Ni J, Wu Y, Yun C, Yu ML and Shen Y*. (2014) cDNA cloning and heterologous expression of an endo-β-1,4-glucanase from the fungus-growing termite Macrotermes barneyi. Arch Insect Biochem Physiol. 86(3):151-64.

5.Watanabe H. and Ni J*. (2013) Functional gene resources from cellulose-feeding insects for novel catalyst in “Biological Conversion of Biomass for Fuels and Chemicals: Explorations from Natural biomass Utilization Systems”. RSC Energy and Environment 2013, p156-76.

6.Ni J*, Tokuda, G. (2013) Lignocellulose–Degrading Enzymes from Termites and Their Symbiotic Microbiota. Biotechnol. Adv. 31(6):838-50.

7.Wu Y, Chi S, Yun C, Shen Y, Tokuda G, Ni J*. (2012) Molecular cloning and characterization of an endogenous digestive β-glucosidase from the midgut of the fungus-growing termite Macrotermes barneyi. Insect Mol Biol.21:604-14.

8.Wu Y, Hou XF, Chi SL and Ni J. * (2012) Isolation and characterization of a Bacillus cereus strain with cellulose-degradation activity from a termite-inhabiting soil. Microbiology China. 39: 638-44.

9.Wu Y, Chi SL and Ni J. (2011) Isolation of a strain of Variovorax sp. R3063 with cellulase activity from termite. Chinese Journal of Applied Entomology. 48: 33-28

10.Ni J.1*, Takehara M and Watanabe H. (2010) Identification of Activity Related Amino Acid Mutations of a GH9 Termite Cellulase. Bioresource Technology. 101:6438-43.

11.Wu L., Liu, B., Hong, Y., Sheng, D., Shen, Y., Ni, J.* (2010) Residue Tyr224 is critical for the thermostability of Geobacillus sp. RD-2 lipase, Biotechnol Lett. 32:107-12.

12.Liu, B., Wu L., Liu, T., Hong, Y., Shen, Y., Ni, J.* (2009) A MOFRL family glycerate kinase from the thermophilic crenarchaeon Sulfolobus tokodaii with unique enzymatic properties, Biotechnol Lett. 31:1937-41.

13.Wang F, Zhang, S. Huang, Q, Shen, Y and Ni, J.* (2009) Advance in genetic manipulation systems of Hyperthermophilic archaea Acta Microbiologica Sinica 49, 1418-23.

14.Huang Q, Shen Y and Ni, J.* (2008) Optimization of selectivr conditions for the selection of uracil auxotrophs of thermophilic archaea Sulfolobus tokodaii. Journal of Shandong University (Natural Science), 43, 6-10.

15.Ni J., Takehara M, Miyazawa M and Watanabe H. (2007) Random exchanges of non-conserved amino acid residues among four parental termite cellulases by family shuffling improved thermostability. Protein Eng. Des. Sel. 20, 535-42.

16.Ni J., Tokuda G, Takehara M and Watanabe H. (2007) Heterologous expression and enzymatic characterization of β-glucosidase from the drywood-eating termite, Neotermes koshunensis. Appl. Entomol. Zool. 42, 457-63.

17.Ni J., Takehara, M and Watanabe H. (2005) Heterologous overexpression of a mutant termite cellulase gene in E.coli by DNA shuffling of four orthologous parental cDNAs, Biosci. Biotechnol. Biochem. 69: 1711-20.


1.Ni J., Shen Y, Zheng T, She Q, Development of expression vector with simvastatin selection marker for hyperthermophilic acidophile.

China invention patent number:ZL2011 1 0020921.0,Certificate No: 109752. Approval date 20121212.

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