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Yulong Shen
Phone number: +86 (531) 8836 2928
Fax number: 
E-mail: yulgshen@sdu.edu.cn
Title: Professor

EDUCATION

Ph.D. 1996 University of Birmingham, Birmingham, UK;

M.S. 1989 Lanzhou University, Lanzhou, Gansu, China;

B.A. 1986 Lanzhou University, Lanzhou, Gansu, China;

WORK EXPERIENCE

2003-the present, Professor, State Key Laboratory of Microbial Technology, Shandong University;

2001-2003, Researcher, Biological information Research Center, National Institute of Advanced Industrial Technology, Japan;

1999-2001, Researcher, Department of Molecular Biology, National Institute of Bioscience and Human-technology, Japan;

1997-1999, Postdoctoral fellow, Department of Citriculture and Biotechnology, National Institute of Fruit Tree Science Japan;

1989-1993, assistant researcher, Lanzhou Institute of Pasturage and Veterinary, CAAS.

RESEARCH INTERESTS

We are mainly interested in the molecular and cell biology of archaea. We use the model thermophilic archaea Sulfolobus to conduct biochemical, structural, and in vivo functional characterization of proteins involved in DNA repair, analysis of ESCRT-III-based cell division process, and investigation into the interaction between host and enveloped virus. The goal is to get a better understanding of DNA damage response, cell division, and host-virus interaction in the archaea and provide insight into evolution of the mechanism between archaea and eukaryotes. We are also interested in the diversity of extremophiles, microbes inhabiting in extreme environments, and the enzymes from them, especially the thermophiles.

RESEARCH PROGRAMS

1. National Science Foundation of China,General Project, 31670061, 2017.01-2020.12

2. National Science Foundation of China,General Project, 31470184, 2015.01-2018.12

3. National Science Foundation of China,General Project, 30870046, 2015.01-2018.12

4. National Science Foundation of China,General Project, 31170072, 2012.01-2015.12

5. National Science Foundation of China,Key Project, 30930002, 2010.01-2013.12

6. National Science Foundation of China,General Project, 30870046, 2009.01-2011.12

PUBLICATIONS

1. Yan Z, Huang Q, Ni J, Shen Y. (2016) Distinct catalytic activity and in vivo roles of the ExoIII and EndoIV AP endonucleases from Sulfolobusislandicus. Extremophiles. 20(5):785-93.

2. Song X, Ni J, Shen Y. (2016) Structure-based genetic analysis of Hel308a in the hyperthermophilicarchaeon Sulfolobusislandicus. J Genet Genomics. 43(6):405-13. PMID: 27317310

3. Song X, Huang Q, Ni J, Shen Y. (2016) Knockout and functional analysis of two DExD/H-box family helicase genes in Sulfolobus islandicus REY15A. Extremophiles. 20(4):537-46.

4. Huang Q, Liu L, Liu J, Ni J, She Q and Shen Y (2015) Efficient 5′-3′ DNA end resection by HerA and NurA is essential for cell viability in the crenarchaeon Sulfolobus islandicus. BMC Molecular Biology 16:2 (DOI:10.1186/s12867-015-0030-z)

5. Huang Q, Li Y, Zeng C, Song T, Yan Z, Ni J, She Q, Shen Y (2015)Genetic analysis of the Holliday junction resolvases Hje and Hjc in Sulfolobus islandicus.Extremophiles. 19:505-14.

6. Han W, Shen Y, She Q (2014) Nanobiomotors of archaeal DNA repair machineries: current research status and application potential. Cell Biosci. 4:32.

7. Liang P, Han W, Huang Q, Li Y, Ni J, She Q Shen Y (2013) Knockouts of RecA-Like Proteins RadC1 and RadC2 Have Distinct Responses to DNA Damage Agents in Sulfolobus islandicus. J Genet Genomics. 40:533-42.

8. Ni J, Wu Y, Yun C, Yu M, Shen Y. (2014) cDNA cloning and heterologous expression of an endo-β-1,4-glucanase from the fungus-growing termite Macrotermes barneyi. Arch Insect Biochem Physiol. 86:151-64.

9. Wang L, Sheng D, Han W, Huang B, Zhu S, Ni J, Li J, Shen Y (2012) Sulfolobus tokodaii RadA paralog, stRadC2, is involved in DNA recombination via interaction with RadA and Hjc. Sci China Life Sci. 55: 261-267.

10. Zheng T, Huang H, Zhang C, Ni J, She Q, Shen Y (2012) Development of simvastatin selection marker for hyperthermophilic acidophile, Applied. Environ. Microbiol. 78:568-574.

11. Hong Y, Chu M, Li Y, Ni J, Sheng D, Hou G, She Q, Shen Y (2012) Dissection of the functional domains of an archaeal Holliday junction helicase, DNA Repair. 11:102-111.

12. Ma X, Hong Y, Han W, Sheng D, Ni, J Hou G &Shen, Y (2011) Single-stranded DNA binding activity of XPBI, but not XPBII, from Sulfolobus tokodaii causes double-stranded DNA melting. Extremophiles. 14:1-10

13. Matsui I, Urushibata Y, Shen Y, Matsui E & Yokoyama H (2011) Novel structure of an N-terminal domain that is crucial for the dimeric assembly and DNA-binding of an archaeal DNA polymerase D large subunit from Pyrococcus horikoshii.FEBS Lett 585:452-8

14. Wei T, Zhang S, Hou L, Ni, J, Sheng D, Hou G & Shen, Y (2011) The carboxyl terminal of the archeal nuclease NurA is involved in the interaction with single-stranded DNA-binding protein and dimer formation. Extremophiles15:11-18.

15. Wu L., Liu, B., Hong, Y., Sheng, D., Shen, Y., Ni, J. (2010) Residue Tyr224 is critical for the thermostability of Geobacillus sp. RD-2 lipase, Biotechnol Lett. 32:107-12.

16. Liu, B., Wu L., Liu, T., Hong, Y., Shen, Y., Ni, J. (2009) A MOFRL family glycerate kinase from the thermophilic crenarchaeon Sulfolobus tokodaii with unique enzymatic properties, Biotechnol Lett. 31:1937-41.

17. Lu S, Li Z, Wang Z, Ma X, Sheng D, Ni J &Shen Y(2008)Spatial subunit distribution and in vitro functions of the novel trimeric PCNA complex from Sulfolobus tokodaii. Biochem Biophys Res Commun 376:369-74.

18. Li Z, Lu S, Hou G, Ma X, Sheng D, Ni J &Shen Y (2008) Hjm/Hel308a DNA helicase from Sulfolobus tokodaii promotes replication fork regression and interacts with Hjc endonuclease in vitro. J Bacteriol 190: 3006–17.

19. Zhang S, Wei W, Hou G, Zhang C, Liang P, Ni J, Sheng D &Shen Y (2008) Archaeal DNA helicase HerA interacts with Mre11 homologue and unwinds blunt-ended double-stranded DNA and recombination intermediates. DNA Repair 7:380–91.

20. Wei T, Zhang S, Zhu S, Sheng D, Ni J &Shen Y (2008) Physical and functional interaction between archaeal single-stranded DNA-binding protein and the 5'-3' nuclease NurA. Biochem Biophys Res Commun 367:523-9.

21. Sheng D, Li M, Jiao J, Ni J &Shen Y (2008) Co-expression with RadA and the characterization of stRad55B, a RadA paralog from the hyperthermophilic crenarchaea Sulfolobus tokodaii. Sci China C Life Sci 51:60-5.

22. Sheng D, Zhu S, Wei T, Ni J & Shen Y (2008) The in vitro activity of a Rad55homologue from Sulfolobus tokodaii, a candidate mediator in RadA-catalyzed homologous recombination. Extremophiles 12:147-57.

23. Hong Y., Wu L., Liu B., Peng C., Sheng D., Ni J, and Shen Y. (2008) Characterization of a glucanphosphorylase from the thermophilic archaeon Sulfolobus tokodaii strain 7. J. Mol. Catal. B: Enzy. 54:27-34

24. Liu B., Hong Y., Wu L., Li Z., Ni J., Sheng D., and Shen Y. (2007) A unique highly thermostable 2-phosphoglycerate forming glycerate kinase from the hyperthermophilic archaeon Pyrococcus horikoshii: gene cloning, expression and characterization. Extremophiles. 11:733-9.

25. Liu B., Li Z., Hong Y., Ni J., Sheng D., and Shen Y. (2006) Cloning, expression and characterization of a thermostable exo-beta-D-glucosaminidase from the hyperthermophilic archaeon Pyrococcus horikoshii. Biotechnol. Lett. 28:1655-1660.

26. Liu B., Ni J., and Shen Y. (2006) Cloning expression and biochemical characterization of a novel diacetylchitobiose deacetylase from the hyperthermophilicarchaeion Pyrococcus horikoshii. ActaMicrobiologicaSinica 46:255-258.

27. Shen Y, Tang XF, Matsui E, Matsui I. (2004) Subunit interaction and regulation of activity through terminal domains of the family D DNA polymerase from Pyrococcushorikoshii. BiochemSoc Trans. 32:245-9.

28. Tang X.-F, Shen Y., Matsui E., MatsuiI. (2004) Domain topology of the DNA polymerase D complex from a hyperthermophilic archaeon Pyrococcus horikoshii. Biochemistry. 43:11818-27.

29. Shen, Y., Tang X.-F., Yokoyama H., Matsui E., and Matsui I. (2004) A 21-aa peptide from the cysteine cluster II of the family D DNA polymerase from Pyrococcus horikoshii stimulates its nuclease activity which is Mre11-like and prefers manganese ion as the co-factor. Nucleic Acids Research 32 (1) 158-68.

30. Shen,Y., Tang X.-F., and Matsui I.(2003)Subunit interaction and regulation of activity through terminal domains of the family D DNA polymerase from Pyrococcus horikoshii. Journal of Biological Chemistry 278:21247-57.

31. Shen, Y., Musti K., Hiramoto M., Kikuchi H., Kawabayashi Y. and Matsui, I. (2001) Invariant Asp1122 and Asp1124 are essential residues for polymerization catalysis of family D DNA polymerase from Pyrococcus horikoshii. Journal of Biological Chemistry 276: 27376-8383.

32. Zheng R., Matsui E., Shen Y., Kishnasastry M., Feng Y., Darnis S., Kawarabayasi Y., Kikuchi H., Harata K., and Matsui I. (2001) The novel function of a short region K253XRXXXD259 conserved in the exonuclease domain of hyperthermostable DNA polymerase I from Pyrococcus horikoshii. Extremophiles 5: 111-7.

33. Shen, Y., Newbury HJ and Ford-Lloyd BV (1998) Identification of taxa in the genus Beta using ITS1 sequence information. Plant Molecular Biology Reporter 16: 147-55.

34. Shen, Y., Ford-Lloyd BV and Newbury HJ (1998) Genetic relationship using both PCR-based and DNA sequencing methods. Heredity 80: 624-32

35. Shen, Y., Newbury HJ and Ford-Lloyd BV (1996) Thetaxonomic characterization of annual Beta germplasm in a genetic resources collection using RAPD makers. Euphytica 91: 205-12

PATENTS

Ni J., Shen Y, Zheng T, She Q, Development of expression vector with simvastatin selection marker for hyperthermophilic acidophile.

China invention patent number:ZL2011 1 0020921.0,Certificate No: 109752. Approval date 20121212.


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