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Doctoral tutor
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Doctoral tutor > microbiology  
Jian Kong
Phone number: 0531 88362318
Fax number: 
E-mail: kongjian@sdu.edu.cn
Title: Professor


2002 – present Professor, State Key Lab of Microbial Technology, SDU

1997 – 2002 Assitant professor, State Key Lab of Microbial Technology, SDU

1992 – 1997 Lecturer, Department of Microbiology, SDU

1986 – 1992 Assitant, Department of Microbiology, SDU

1999 – 2000 Visiting Scholar, Royal Agricultural and Veterinary University, Denmark

1997 – 2001 Ph.D., School of Life Science, Shandong University

1988 –1992 M.Sc., Department of Microbiology, Shandong University

1982 –1986 B.Sc., Department of Microbiology, Shandong University(SDU)


1. Lactic acid bacteria and their bacteriophages

2. Bioactive components produced by lactic acid bacteria

3. Development of genetic tools for lactic acid bacteria

4. Lactic acid bacteria used as starter cultures, probiotics, feedstuff additives or bacterial inoculants


1.Public Service Sectors (Agriculture) Special and Scientific Research Projects (201503134) : Development o of bacterial inoculants in silage fermention for impoving the silage quility. 2015.1~2019.12.

2.National Natural Science Foundation of China (NSFC) (31471715): Characterization of a enzymic defence against the peroxide stress in Streptococcus thermophiles by comparative genome analysis. 2015.1-2019.12 .

3.National Sci-Tech Support Program of China (2012BAD39B01) : Development of functional probiotics for the intestinal health and nutrient regulation of chicken. 2012.1-2016.12.

4.National Natural Science Foundation of China (NSFC) (31271905): Functional analysis of the lysis mechinsm of the Endolysin and Holin coding by the temperate bacteriophageФYB5 in Lactobacillus fermtrum 2013.1-2016.12 .

5.HI-Tech Research and Development Program of China(2011AA100902): Metabolic engineering and metabolic regulation in Lactic acid bacteria. 2011.01—2015.12.

6.National Natural Science Foundation of China(31070091): Construction of a synthetic promoter library and optimization the pyruvate flux from lactic acid to diacelyl. 2011.01-2013.12.

7.National Natural Science Foundation of China (30870023). Surface Display system in lactic acid bacteria and potential application as delivery vehicles for antigens. 2008.01-2011.12

8.National Natural Science Foundation of China(30570043): Lactic acid bacteria as live vechiles for development of oral vacinnes against PCV2 infection. 2006.01-2008.12

9.Science and High- Techology project of Shandong Province (2011GGE27088) : Selection and high density fermentation of lactic acid bacteria. 2011.01-2012.12

10.National Sci-Tech Support Program of China(2006AA10Z321): Construction of food-grade expression in Lactic acid bacteria. 2006.01-2010.10.

11.National Sci-Tech Support Program of China (2006AA10Z344). Development of the starter cultures of lactic acid bacteria. 2006.01-2009.10.

12.National Sci-Tech Support Program of China (2007AAZ10Z335). Probiotics screening and functional analysis. 2007.01-2010.12


1.Guo, T., Xin, Y., Zhang, C., Ouyang, X., & Kong, J. The potential of the endolysin Lysdb from Lactobacillus delbrueckii phage for combating Staphylococcus aureus during cheese manufacture from raw milk. Appl Microbiol Biotechnol, (2016) 100:3545–3554.

2.Ting Wang, Zhenshang Xu, Shiyi Lu, Min Xin, Jian Kong*. Effects of glutathione on acid stress resistance and symbiosis between Streptococcus thermophilus and Lactobacillus delbrueckii subsp. Bulgaricus. International Dairy Journal 61 (2016) 22~28

3.Tingting Guo, Chenchen Zhang, Yongping Xin, Min Xin, Jian Kong. A novel chimeric prophage vB_LdeS phiJB from commercial Lactobacillus delbrueckii subsp. Bulgaricus. Journal of Industrial Microbiology & Biotechnology, 2016. 43(5), 681-689

4.Wenwei Lu , Wentao Kong , Pu Yang, Jian Kong*. A one-step PCR-based method for specific identification of 10 common lactic acid bacteria and Bifidobacterium in fermented milk. International Dairy Journal. 2015, 41: 7~12. IF 2.433

5.W. W. Lu, Y. Wang, T. Wang, J. Kong*. The Global Regulator CodYst in Streptococcus thermophillus Controls the Metabolic Network for Escalating Growth in the Milk Environment. Applied and Environmental Microbiology. 2015, 81( 7); 2349-2358.

6.Ting Wang, Wenwei Lu, Shiyi Lu, Jian Kong*. Protective role of glutathione against oxidative stress in Streptococcus thermophilus. International Dairy Journal. 2015, 45: 41~47.

7.Tingting Guo, Chenchen Zhang, Wei Liu, ShaohuaWang, Jian Kong*. Functional analysis of the N-terminal region of endolysin Lyb5 encoded by Lactobacillus fermentum bacteriophage φPYB5. International Journal of Food Microbiology. 2015, 203: 1–7.

8.Yi Xu, TingWang, Jian Kong*, Hui-Li Wang. Identification and functional characterization of AclB, a novel cell-separating enzyme from Lactobacillus casei. International Journal of Food Microbiology. 2015, 203: 93–100.

9.Chenchen Zhang, Yongping Xin, Yue Wang, Tingting Guo, Shiyi Lu, Jian Kong*. Identification of a Novel Dye-Decolorizing Peroxidase, EfeB, Translocated by a Twin-Arginine Translocation System in Streptococcus thermophilus CGMCC 7.179. Applied and Environmental Microbiology. 2015, 81(18): 6108~6119.

10.Zhang, C., Guo, T., Xin, Y., Gao, X., Kong, J. Catabolite responsive element deficiency of xyl operon resulting in carbon catabolite derepression in Lactobacillus fermentum 1001. J Appl Microbiol, 2015.120( 1): 126–137.

11.Zhilan Sun, Jian Kong*, Shumin Hu, Wentao Kong, Wenwei Lu, Wei Liu. Characterization of a S-layer protein from Lactobacillus crispatus K313 and the domains responsible for binding to cell wall and adherence to collagen. Appl Microbiol Biotechnol, 2013. 97:1941–1952.

12.Longyun Fu, Jian Kong*, Zhilan Sun,, Li Zhang, Xia Zhang, Tingting Guo. Enhancing the oxidative resistance of yoghurt starter bacteria with heterologous catalase expression in Streptococcus thermophilus. International Dairy Journal 30 (2013) 68-72.

13.Wenwei Lu, Jian Kong, Wentao Kong. Construction and Application of a Food-Grade Expression System for Lactococcus lactis. Molecular biotechnology. 2013, 54:170–176

14. Tingting Guo, Shumin Hu, Jian Kong. Functional Analysis and Randomization of the Nisin-Inducible Promoter for Tuning Gene Expression in Lactococcus lactis. Current Microbiology. 2013 ;66(6):548-545.

15.Yi Xu, Jian Kong and Wentao Kong. Improved membrane protein expression in Lactococcus lactis by fusion to Mistic. Microbiology (2013), 159, 1002–1009.

16.Yi Xu, Jian Kong. Construction and Potential Application of the Controlled Autolytic Systems for Lactobacillus casei in Cheese Manufacture。J Food Prot. 2013 ;76(7):1187-93.

17.Li H, Ma ML, Xie HJ, J Kong* .Biosafety evaluation of bacteriophages for treatment of diarrhea due to intestinal pathogen Escherichia coli 3-2 infectionof chickens. World J Microbiol Biotechnol, 2012, 28:1–6.

18.Tingting Guo, Jian Kong*, Li Zhang, Chenchen Zhang, Shumin Hu. Fine Tuning of the Lactate and Diacetyl Production through Promoter Engineering in Lactococcus lactis. PLoS ONE. 2012, 7(4):1-10.

19.Zhilan Sun, Lihua Huang, Jian Kong*, Shumin Hu, Xiaowei Zhang, Wentao Kong. In vitro evaluation of Lactobacillus crispatus K313 and K243: High-adhesion activity and anti-inflammatory effect on Salmonella braenderup infected intestinal epithelial cell. Veterinary Microbiology. 2012, 159 :212–220.

20.Wentao Kong,Jian Kong*,Shumin Hu,Wenwei Lu, Ke Wang, Mingjie Ji. Enhanced expression of PCV2 capsid protein in Escherichia coli and Lactococcus lactis by codon optimization. World J Microbiol Biotechnol, 2011, 27:651–657.

21.Xiuhong Zhang, Shaohua Wang, Tingting Guo, Jian Kong*. Genome analysis of Lactobacillus fermentum temperate bacteriophage ФPYB5. International Journal of Food Microbiology. 2011, 144: 400–405.

22.Yi Xu , Yan Niu, Jian Kong*.Heterologous overexpression of a novel delta-4 desaturase gene from the marine microalga Pavlova viridis in Escherichia coli as a Mistic fusion. World J Microbiol Biotechnol. 2011,27:2931–2937.


1. Jian Kong et al., A Paenibacillus illinoisensis strain with beta- and alpha-galactosidase :Patent Number :

NL 201210580938.6

2. Jian Kong et al., A method for mixing cultural of Lactobacillus and Bacillus species. Patent Number:NL201210581009.7

3. Jian Kong et al., The gene encoded for C20 enlongase in pavlova viridis and its application. Patent Number: ZL20071007100152165

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