硕士生导师
符军
作者:   时间:2017-12-12   点击数:

符军

电 话:86-0532-67720928传 真:E-Mail:fujun@sdu.edu.cn

教育背景

1987 – 1991 西北农林科技大学(学士)

1998 – 2001 德累斯顿工业大学(硕士)

2004 – 2012 德累斯顿工业大学(博士)

工作经历

1991 – 1998 西北农林科技大学

2002 – 2004 基因桥公司(Gene Bridges GmbH)

2004 – 2014 德累斯顿工业大学生物技术中心

2014 – 微生物技术国家重点实验室,生命科学学院,山东大学,

研究方向

1. DNA同源重组机理研究,基因克隆技术开发。

2. 基因打靶技术和BAC转基因技术研究;转基因动物。

3. 细菌次生代谢产物基因簇的克隆,修饰和异源表达;药物开发。

4. 病毒基因组DNA文库构建和重组疫苗开发。

主持课题

1. 应用自身噬菌体重组蛋白建立枯草芽孢杆菌DNA同源重组系统(国家自然科学基金面上项目, 31570094)2016年1月1日-2019年12月31日

2. 磁小体基因簇的克隆及应用于肿瘤靶向治疗(国家国际科技合作专项, 2015DFE32850)2015年4月1日-2018年3月31日

代表性论文

1. Wenli Zhang,Jun Fu, Jing Liu, Hailong Wang, Maren Schiwon, Sebastian Janz, Lukas Schaffarczyk, Lukas von der Goltz, Eric Ehrke-Schulz, Johannes Dörner, Manish Solanki, Philip Boehme, Thorsten Bergmann, Andre Lieber, Chris Lauber, Andreas Dahl, Andreas Petzold, Youming Zhang, A. Francis Stewart and Anja Ehrhardt (2017) “An Engineered Virus Library as a Resource for the Spectrum-wide Exploration of Virus and Vector Diversity” Cell Reports. 19(8):1698-1709.

2. Hailong Wang, Zhen Li, Ruonan Jia, Yu Hou, Jia Yin, Xiaoying Bian, Aiying Li, Rolf Müller, A Francis Stewart,Jun Fu& Youming Zhang (2016) "A concerted pipeline for direct cloning of biosynthetic gene clusters and engineering for heterologous expression" Nature Protocols 11(7): 1175-90.

3. Oliver Baker, Ashish Gupta, Mandy Obst, Youming Zhang, Konstantinos Anastassiadis,Jun Fuand A. Francis Stewart (2016) “RAC-tagging: Recombineering And Cas9-assisted targeting for protein tagging and conditional analyses” Scientific Reports 2016 May 24;6:25529. doi: 10.1038/srep25529.

4. Yin, J., Zhu, H., Xia, L., Ding, X., Hoffmann, T., Hoffmann, M., Bian, X., Müller, R.,Fu, J., Stewart, A.F., Zhang, Y. (2015) “A new recombineering system for Photorhabdus and Xenorhabdus” Nucleic Acids Research 2015 Mar 31;43(6):e36. doi: 10.1093/nar/gku1336

5. Wang, H., Bian, X., Xia, L., Ding, X., Müller, R., Zhang, Y.,Fu, J.and Stewart, A. F. (2014) " Improved seamless mutagenesis by recombineering using ccdB for counterselection " Nucleic Acids Research 2013 Dec 24. [Epub ahead of print]

6. Filipczyk, A., Gkatzis, K.,Fu, J., Lickert, H., Anastassiadis, K. and Schroeder, T. (2013) " Biallelic Expression of Nanog Protein in Mouse Embryonic Stem Cells" Cell Stem Cell 13:12-13

7. Rostovskaya, M.,Fu, J., Obst, M., Baer, I., Weidlich, S., Wang, H., Smith, A., Anastassiadis, K. and Stewart, A. F. (2012) “Transposon-Mediated BAC Transgenesis in Human ES Cells” Nucleic Acids Research, 2012 Jun 30. [Epub ahead of print] doi: 10.1093/nar/gks643

8.Fu, J., Bian, X., Hu, S., Wang, H., Huang, F., Seibert, P., Plaza, A., Xia, L., Müller, R., Stewart, A. F. and Zhang, Y. (2012) "Full-length RecE enhances linear-linear homologous recombination and facilitates direct cloning for bioprospecting.” Nature Biotechnology 30: 440-446.

9. Bird, A. W., Erler, A.,Fu, J., Maresca, M., Heriche, J. K., Zhang, Y., Hyman, A. A. and Stewart, A. F. (2011) "High-efficiency counterselection recombineering for site-directed mutagenesis in bacterial artificial chromosomes." Nature Methods 9:103-109.

10. Nedelkova, M., Maresca, M.,Fu, J.,Rostovskaya, M., Thiede, C., Anastassiadis, K., Sarov, M. and Stewart, A. F. (2011). "Targeted isolation of genomic regions by recombineering." Nucleic Acids Research 39:e137.

11. Daniel, K., Lange, J., Hached, K.,Fu, J., Anastassiadis, K., Roig, I., Cooke, H. J., Stewart, A. F., Wassmann, K., Jasin, M., Keeney, S. and Toth, A. (2011). "Meiotic homologue alignment and its quality surveillance are controlled by mouse HORMAD1." Nature Cell Biology 13:599-610.

12. Skarnes, W. C., Rosen, B., West, A. P., Koutsourakis, M., Bushell, W., Iyer, V., Cox, R., Jackson, D., Severin, J., Biggs, P., Thomas, M., Mujica, A., Harrow, J.,Fu, J.,Nefedov, M., de Jong, P., Stewart, A. F. and Bradley, A. (2011). "A conditional knockout resource for genome-wide analysis of mouse gene function." Nature 474: 337-42.

13.Fu, J., Teucher, M., Anastassiadis, K., Skarnes, W. and Stewart, A. F. (2010). "A Recombineering pipeline to make conditional targeting constructs." Methods in Enzymology 477C: 125-144.

14.Fu, J., Wenzel, S. C., Perlova, O., Wang, J., Gross, F., Tang, Z., Yin, Y., Stewart, A. F., Müller, R. and Zhang, Y. (2008). "Efficient transfer of two large secondary metabolite pathway gene clusters into heterologous hosts by transposition." Nucleic Acids Research 36: e113.

专利:

1. TU, Qiang; MÜLLER, Rolf; STEWART, Francis;FU, Jun; ZHANG, Youming. “High efficiency of electrocompetent cells prepared at room temperature” PCT/EP2013/071546

2.FU, Jun; ZHANG, Youming; STEWART, Francis. “Direct cloning of DNA fragment from genomic DNA without library construction and screening” PCT/IB2011/052549

3. ZHANG, Youming;FU, Jun; BIAN, Xiaoying; STEWART, Francis; ZIPS, Daniel; MÜLLER, Rolf “Heterologous expression of PKS/NRPS gene cluster and targeted anticancer drug delivery.” PCT/IB2010/055923

4. MARESCA, Marcello; ERLER, Axel, Steffen;FU, Jun; SEIBERT, Philipp, Martin; STEWART, Adrian, Francis; ZHANG, Youming “Method of nucleic acid recombination” PCT/IB2009/000488

5. ZHANG, Youming; MÜLLER, Rolf; STEWART, Francis; GROSS, Frank; WENZEL,Silke, C.;FU, Jun“Methods for heterologous expression of secondary metabolites” PCT/IB2005 /003650; EP1809749 (2007); US20090298117 (2009)

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