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微生物学

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申玉龙

0531-88362903 yulgshen@sdu.edu.cn

教育背景

博士  1993.8-1996.12    University of Birmingham (UK)    细胞与分子生物学

硕士  1986.9-1989.6           兰州大学                                   植物学

本科  1982.9-1986.6           兰州大学                                   植物学

工作经历    

2003.11-现在     山东大学微生物技术国家重点实验室  教授,博导

1999.4-2003.10  日本通产省产业技术综合研究所   研究员

1997.1-1999.3    日本农水省果树研究所  博士后

1989.7-1993.8    中国农业科学院兰州畜牧研究所  助理研究员

科研方向

1. 极端嗜热古菌DNA修复蛋白的生化性质、结构及体内外功能鉴定

2. 硫化叶菌细胞分裂机制及其病毒的出芽机制研究

3. 极端环境微生物资源及极端酶的研究与利用


课题组主要研究古菌的分子与细胞生物学,利用模式古菌(硫化叶菌)开展古菌DNA修复蛋白的生化性质、结构与体内功能的鉴定,分析以真核生物ESCRT-III 同源蛋白为基础的细胞分裂机制,解析衣包病毒在冰岛硫化叶菌宿主的出芽机制。研究目标主要是揭示这些过程的分子机制,为研究从古菌到真核生物的进化提供基础。同时,我们也对极端环境微生物的多样性及极端酶开展研究。

研究课题

1. 2017.01-2020.12  冰岛硫化叶菌新型ATP酶PINA的生化性质与结构及其在同源重组修复中的作用研究,国家自然科学基金面上项目

2. 2015.01-2018.12  硫化叶菌Holliday junction解离酶Hje的体内功能与作用机制研究,国家自然科学基金面上项目

3. 2015.01-2018.12 冰岛硫化叶菌膜泡转运蛋白ESCRT-III同源蛋白的遗传分析,国家自然科学基金面上项目

4. 2012.01-2015.12  超嗜热古菌Sulfolobusislandicus 核酸切除修复途径研究,

国家自然科学基金面上项目

5. 2010.01-2013.12  超嗜热古菌DNA重组修复解旋酶和核酸酶的作用机制及体内功能,国家自然科学基金重点项目

6. 2009.01-2011.12 古菌Hjm解旋酶参与复制叉回退和Holliday junction加工的机制,国家自然科学基金面上项目

研究成果

1. Yan Z, Huang Q, Ni J, Shen Y. (2016) Distinct catalytic activity and in vivo roles of the ExoIII and EndoIV AP endonucleases from Sulfolobu sislandicus. Extremophiles. 20(5):785-93.

2. Song X, Ni J, Shen Y.(2016) Structure-based genetic analysis of Hel308a in the hyperthermophilicarchaeon Sulfolobus islandicus.J Genet Genomics.43(6):405-13. PMID: 27317310

3. Song X, Huang Q, Ni J, Yu Y, Shen Y.(2016) Knockout and functional analysis of two DExD/H-box family helicase genes in Sulfolobus islandicus REY15A.Extremophiles. 20(4):537-46.

4. Huang Q, Liu L, Liu J, Ni J, She Q and Shen Y (2015) Efficient 5′-3′ DNA end resection by HerA and NurA is essential for cell viability in the crenarchaeonSulfolobus islandicus. BMC Molecular Biology 16:2 (DOI:10.1186/s12867-015-0030-z)

5. Huang Q, Li Y, Zeng C, Song T, Yan Z, Ni J, She Q, Shen Y (2015)Genetic analysis of the Holliday junction resolvases Hje and Hjc in Sulfolobus islandicus.

Extremophiles. 19:505-14.

6. Han W, Shen Y, She Q (2014) Nanobiomotors of archaeal DNA repair machineries: current research status and application potential.Cell Biosci. 4:32.

7. Liang P, Han W, Huang Q, Li Y, Ni J, She Q, Shen Y (2013) Knockouts of RecA-Like Proteins RadC1 and RadC2 Have Distinct Responses to DNA Damage Agents in Sulfolobus islandicus. J Genet Genomics. 40:533-42.

8. Ni J, Wu Y, Yun C, Yu M, Shen Y. (2014) cDNA cloning and heterologous expression of an endo-β-1,4-glucanase from the fungus-growing termite Macrotermesbarneyi.Arch Insect Biochem Physiol. 86:151-64.

9. Wang L, Sheng D, Han W, Huang B, Zhu S, Ni J, Li J, Shen Y (2012) Sulfolobus tokodaii RadA paralog, stRadC2, is involved in DNA recombination via interaction with RadA and Hjc. Sci China Life Sci. 55: 261–267.

10. Zheng T, Huang H, Zhang C, Ni J, She Q, Shen Y (2012) Development of simvastatin selection marker for hyperthermophilic acidophile, Applied. Environ. Microbiol. 78:568-574.

11. Hong Y, Chu M, Li Y, Ni J, Sheng D, Hou G, She Q, Shen Y (2012) Dissection of the functional domains of an archaeal Holliday junction helicase, DNA Repair.  11:102-111.

12. Matsui I, Urushibata Y, Shen Y, Matsui E, Yokoyama (2011) Novel structure of an N-terminal domain that is crucial for the dimeric assembly and DNA-binding of an archaeal DNA polymerase D large subunit from Pyrococcus horikoshii.FEBS Lett. 585(3):452-8.

13. Ma X, Hong Y, Han W, Sheng D, Ni, J Hou G & Shen, Y (2011) Single-stranded DNA binding activity of XPBI, but not XPBII, from Sulfolobus tokodaii causes double-stranded DNA melting. Extremophiles. 14:1-10.

14. Wei T, Zhang S, Hou L, Ni, J, Sheng D, Hou G & Shen, Y (2011) The carboxyl terminal of the archeal nuclease NurA is involved in the interaction with single-stranded DNA-binding protein and dimer formation. Extremophiles 15:11-18.

15. Lei Wu,Bo Liu,Ye Hong,Duohong Sheng,Yulong Shen,Jinfeng Ni. (2009) Residue Tyr224 is critical for the thermostability of Geobacillus sp. RD-2 lipase, Biotechnol Lett. 32:107-12.  

16. Bo Liu, Lei Wu, Tianming Liu, Ye Hong,Yulong Shen, Jinfeng Ni. (2009) A MOFRL family glycerate kinase from the thermophiliccrenarchaeon Sulfolobu stokodaii with unique enzymatic properties, Biotechnol Lett. Online Aug. 19, DOI 10.1007/s10529-009-0089-z.

17. 王飞雁、张松涛、黄奇洪、申玉龙、倪金凤,(2009) 超嗜热古菌遗传转化系统研究进展。微生物学报49,1418-1423。

18. 黄奇洪、申玉龙、倪金凤,超嗜热古菌尿嘧啶营养缺陷型筛选条件的最适化及初步筛选 山东大学学报 2008,43:6-10。

19. Liu T, Shen Y, Liu Q, Liu B (2008) The unique Entner-Doudoroff (ED) glycolysis pathway of glucose in archaea--a review.Acta Microbiologica Sinica. 48:1126-31

20. Lu S, Li Z, Wang Z, Ma X, Sheng D, Ni J & Shen Y (2008)Spatial subunit distribution  and in vitro functions of the novel trimeric PCNA complex from Sulfolobustokodaii. Biochem Biophys Res Commun 376:369-74.

21. Li Z, Lu S, Hou G, Ma X, Sheng D, Ni J & Shen Y(2008) Hjm/Hel308a DNA helicase fromSulfolobus tokodaiipromotes replication fork regression and interacts with Hjc endonuclease in vitro. J Bacteriol. 190: 3006–17.

22. Zhang S, Wei W, Hou G, Zhang C, Liang P, Ni J, Sheng D & Shen Y (2008) Archaeal DNA helicase HerA interacts with Mre11 homologue and unwinds blunt-ended double-stranded DNA and recombination intermediates. DNA Repair. 7:380–91.

23. Wei T, Zhang S, Zhu S, Sheng D, Ni J &Shen Y (2008) Physical and functional interaction between archaeal single-stranded DNA-binding protein and the 5'-3' nuclease NurA. Biochem Biophys Res Commun. 367:523-9.

24. Sheng D, Li M, Jiao J, Ni J &Shen Y(2008) Co-expression with RadA and the characterization of stRad55B, a RadA paralog from the hyperthermophilic crenarchaea Sulfolobu stokodaii. Sci China C Life Sci. 51:60-5.

25. Sheng D, Zhu S, Wei T, Ni J & Shen Y (2008) The in vitro activity of a Rad55 homologue fromSulfolobus tokodaii, a candidate mediator in RadA-catalyzed homologous recombination. Extremophiles. 12:147-57.

26. Hong Y., Wu L., Liu B., Peng C., Sheng D., Ni J, and Shen Y. (2008) Characterization of a glucanphosphorylase from the thermophilic archaeon Sulfolobus tokodaiistrain 7. J. Mol. Catal. B: Enzy. 54:27-34

27. Liu B., Hong Y., Wu L., Li Z., Ni J., Sheng D., and Shen Y. (2007) A unique highly thermostable 2-phosphoglycerate forming glycerate kinase from the hyperthermophilic archaeon Pyrococcus horikoshii: gene cloning, expression and characterization. Extremophiles. 11:733-9.

28. Liu B., Li Z., Hong Y., Ni J., Sheng D., and Shen Y. (2006) Cloning, expression and characterization of a thermostable exo-beta-D-glucosaminidase from the hyperthermophilic archaeon Pyrococcus horikoshii. Biotechnol. Lett. 28:1655-1660.

29. Liu B., Ni J., and Shen Y. (2006) Cloning expression and biochemical characterization of a novel diacetylchitobiose deacetylase from the hyperthermophilicarchaeionPyrococcus horikoshii. Acta Microbiologica Sinica 46:255-258.

30. Shen, Y., Tang X.-F., Yokoyama H., Matsui E., and Matsui I. (2004) A 21-aa peptide from the cysteine cluster II of the family D DNA polymerase from Pyrococcushorikoshii stimulates its nuclease activity which is Mre11-like and prefers manganese ion as the co-factor. Nucleic Acids Research. 32: 158-168.

31. Shen Y, Tang XF, Matsui E, Matsui I. (2004) Subunit interaction and regulation of activity through terminal domains of the family D DNA polymerase from Pyrococcus horikoshii. Biochem Soc Trans. 32:245-9.

32. Shen Y, Tang XF, Matsui I. (2003) Subunit interaction and regulation of activity through terminal domains of the family D DNA polymerase from Pyrococcus horikoshii. J Biol Chem. 278(23):21247-57.

33. Tang X.-F, Shen Y., Matsui E., Matsui I. (2004) Domain topology of the DNA polymerase D complex from a hyperthermophilic archaeon Pyrococcushorikoshii. Biochemistry. 43:11818-27.

34. Shen Y, Musti K, Hiramoto M, Kikuchi H, Kawarabayashi Y, Matsui I. (2001) Invariant Asp-1122 and Asp-1124 are essential residues for polymerization catalysis of family D DNA polymerase from Pyrococcushorikoshii.J Biol Chem. 276(29):27376-83.

35. Zheng R., Matsui E., Shen Y., Kishnasastry M., Feng Y., Darnis S., Kawarabayasi Y., Kikuchi H., Harata K., and Matsui I. (2001) The novel function of a short region K 253 XRXXXD 259 conserved in the exonuclease domain of hyperthermostable DNA polymerase I from Pyrococcus horikoshii. Extremophiles 5:111-7.

36. Shen Y., Newbury H. J., and Ford-Lloyd B. V. (1998) Identification of taxa in the genus Beta using ITS1 sequence information. Plant Mol. Biol. Rep. 16: 147-55.

37. Shen Y., Ford-Lloyd B. V., and Newbury H. J. (1998) Genetic relationship using both PCR-based and DNA sequencing methods. Heredity 80: 624-32.

38. Shen Y., Newbury H. J., and Ford-Lloyd B. V. (1996) The taxonomic characterization of annual Beta germplasm in a genetic resources collection using RAPD makers. Euphytica 91: 205-12.

专利  

倪金凤,申玉龙,郑涛等 一种基于抗生素标记的超嗜热泉古菌表达载体及其应用,中国发明专利号2011100209210



 

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